FIG. 1.

PBMC were isolated from whole blood by use of Leucosep tubes, and the quality of the procedure was assessed. (A) Pie chart showing mean (range) proportions of granulocytes (50.3% [28.4% to 78.9%]), lymphocytes (40.4% [17.0% to 58.7%]), and monocytes (9.3% [2.0% to 42.5%]) from 52 whole-blood samples before processing. (B) Pie chart showing mean (range) proportions of granulocytes (8.8% [1.2% to 45.8%]), lymphocytes (69.9% [29.4% to 92.6%]), and monocytes (21.4% [4.9% to 56.3%]) from 52 samples after PBMC processing. (C) Bar chart showing the mean concentration of platelets (10⁶) in whole blood (1,034 [35 to 223]) or after processing (12 [0 to 100]). The effect of processing and cryopreservation on the immune phenotype was assessed and compared to peripheral blood concentrations. (D) Box and whisker plots showing the median and 10th and 90th percentiles for major immune cell subsets in 40 normal healthy individuals. A statistically significant difference was observed for the percentage of B cells (P < 0.001). (E) Box and whisker plots showing the median and 10th and 90th percentiles for major immune cell subsets in 28 HIV-1-positive individuals. A statistically significant decrease was observed for the percentage of B cells (P = 0.007).