TABLE 2.
PBMC quality measures
| Parameter | Target | Impact | Reference(s) |
|---|---|---|---|
| Analytical parameters | |||
| Granulocytes (%) | 3.0 ± 2.7 | High | 40 |
| Lymphocytes (%) | 60-70 (with Leucosep tubes, 87.6 ± 4.3) | High | 24, 40 |
| Monocytes (%) | 9.1 ± 3.8 | Low | 40 |
| Platelets (%) | 0-88.2 (<0.5% of original content) | High | 24 |
| Viability (%) | >95 | Medium | 24 |
| Yield (cells/ml whole blood) | 1 × 106-2 × 106 | Medium | 24 |
| Postanalytical parameters | |||
| ICS | Various targets, including low background, consistently strong positive controls, and preserved antigen-specific responses | High | 1, 9, 28 |
| Precision | Code of variation of <5% | Medium | |
| Long-term storage in liquid nitrogen (yr) | <10 is optimal, but indefinite storage has been suggested | Low | 12, 22 |
| Transport | Maintain cold chain | High | 13 |
| Overnight viability (%) | >70-75 | High | 46 |
| Overnight recovery (%) | >50 | High |