TABLE 1.
Primers used to generate agfA::eso9 and agfA::eso11
| Primera | Sequenceb (5′-3′) |
|---|---|
| eso9-A | GACTGGATCCTGTCCGTTATTTCACAAG |
| eso9-B | CTGAGTAATCCACATAAGAAGAGAAGCTGCTTTACGGGCATCGCTTTG |
| eso9-C | CTTCTTATGTGGATTACTCAGTGCGCTGCGGGCCAGGGTGCGGATAAC |
| eso9-D | GACTCTGCAGAGGGTTCGTTTAATGTGA |
| eso11-A | GACTGGATCCTGTCCGTTATTTCACAAG |
| eso11-B | GAAGCACTGAGTAATCCACATAAGAAGAGACGCTGCTTTACGGGCATCGCTTTG |
| eso11-C | CTTCTTATGTGGATTACTCAGTGCTTCCTTGCTGCGGGCCAGGGTGCGGATAAC |
| eso11-D | GACTCTGCAGAGGGTTCGTTTAATGTGA |
a
Primers used to generate agfA::eso, as noted in Fig. 1.
b
Boldface nucleotides encode the NY-ESO-1 epitopes.