FIG. 5.

Map of CpP2 epitopes recognized by human serum IgG antibodies. A series of 33 overlapping biotinylated peptides (15-mers, 12-residue overlap) were synthesized on the basis of the CpP2 cloned sequence. Peptides (100 ng/well) were incubated in streptavidin-coated 96-well microtiter plates (200 ng protein/well) overnight at 4°C. The plates were then washed and incubated with 1:100-diluted Haiti blood donor sera for 2 h at room temperature. The plates were washed and then developed by using peroxidase-labeled goat anti-human IgG and ABTS, as described in Materials and Methods. The CpP2 peptide responses are expressed as a percentage of the value for the positive-control peptide.