FIG. 1.

Representative gels to show signals obtained for SRA- and TgSGP-PCRs from corresponding templates. No TgSGP signal was obtained from any of the FTA cards spotted with patient blood from Lwala or Kaliua. Lanes: M, 0.2-kb to 10-kb Smart ladder; M', 100-bp ladder (NEB); 1 to 4, samples from patients at Kaliua (11001, 11006, 11012 and 1106); 5 to 7, samples from T. b. gambiense patients from Omugo (OM187, OM197 and OM203); 8 to 10, laboratory strains GVR35 (T. b. brucei), UGA015 (T. b. rhodesiense), and Eliane (T. b. gambiense), respectively; 11, negative control (where water was added in place of the template).