TABLE 1.
Samples for which either the SRA-PCR, SRA-LAMP or RIME-LAMP were negativea
| Sample site or strain | No. of samples positive by indicated test/total no. of samples |
|||
|---|---|---|---|---|
| Stained smear | SRA-PCR | SRA-LAMP | RIME-LAMP | |
| Kaliua | 25/25 | 5/25 | 22/25 | 23/25 |
| Lwala | 9/10 | 3/10 | 5/10 | 6/10 |
| Omugo controls | ND* | 0/3 | 0/3 | 3/3 |
| T. b. rhodesiense lab strain | NA | 1/1 | 1/1 | 1/1 |
| T. b. brucei lab strain | NA | 0/1 | 0/1 | 1/1 |
| T. b. gambiense lab strain | NA | 0/1 | 0/1 | 1/1 |
a
Trypanosomes were demonstrated by stained smears in all samples except sample 12008 from Lwala, indicating the presence of DNAs that should have been detected by molecular methods. Only 93 samples had positive results for each of the three tests. All patient samples were negative by TgSGP-PCR, except the three from Omugo, northwestern Uganda. NA, not applicable; ND, not done. An asterisk indicates samples that were positive by the hematocrit centrifugation technique described by Woo et al. (17) routinely used at Omugo Health Center.