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. 2010 Apr 14;48(6):2130–2139. doi: 10.1128/JCM.01940-09

TABLE 1.

Genetic and serological characteristics of Staphylococcus aureus isolates from bovine milk and humansa

MLST
 Spa type Coagulase serotype coa PCR-RFLP genotype PFGE pulsotype(s) Toxin gene profile No. of RUsb in SIRU locus:
 SCCmec type No. of locations where foundc Prefecture(s) where found Source(s)
CC ST 1 5 7 13 15 21
5 5 t001 II 676H B6 hlg, lukE/lukD 2 30< 1 3 999 9 II 1 Tochigi Human wound
5 5 t002 II 676B D hlg, lukE/lukD 2 3 1 3 1 9 II 1 Unknown Bovine milk
5 5 t179 II 676B B1, B2, B3 hlg, lukE/lukD 2 3 1 3 1 9 3 Fukuoka Bovine milk
5 5 t375 II 676B E1, E2 hlg, lukE/lukD 2 2 1 3 999 9 II 1 Unknown Bovine milk
5 5 t375 II 676B E3 hlg, lukE/lukD 2 2 1 3 999 9 II 1 Ibaraki Human pus
5 5 t5259 II 676B B4, B5 hlg, lukE/lukD 2 3 1 3 1 6 1 Fukuoka Bovine milk
6 1362 t701 IV 676D Q11 hlg, lukE/lukD 2 7 999 4 1 9 1 Ishikawa Bovine milk
6 6 t2360 IV 676D Q10 hlg, lukE/lukD 2 7 999 4 1 10 1 Tottori Bovine milk
7 789 t091 III 757B H1, H2 hlg, lukE/lukD, sagB, sagD 999 999 3 2 3 9 2 Iwate, Saga Bovine milk
8 8 t008 III 595B G2 hlg, lukE/lukD 3 3 1 0 1 9 1 Iwate Bovine milk
8 8 t008 III 595C G6 hlg, lukE/lukD 3 3 2 0 1 9 1 Hokkaido Bovine milk
8 8 t008 III 352A G3 hlg, lukE/lukD 3 3 3 0 1 9 1 Ishikawa Bovine milk
8 8 t4133 III 514A G1 hlg, lukE/lukD 3 3 3 0 1 9 1 Gunma Bovine milk
8 72 t4359 V 676F M hlg, lukE/lukD 3 9 999 3 7 4 1 Ishikawa Bovine milk
8 630 t377 III 595B G4, G5 hlg, lukE/lukD 4 6 2 0 1 7 1 Iwate Bovine milk
12 12 t160 VII 676D R5 hlg, lukE/lukD 1 7 1 3 999 6 1 Chiba Bovine milk
12 12 t160 VII 676D R2 hlg, lukE/lukD 1 7 1 3 1 6 1 Saga Bovine milk
12 12 t3418 VII 676D R3 hlg, lukE/lukD 1 7 1 4 1 5 1 Tochigi Bovine milk
12 1369 t160 VII 676D R4 hlg, lukE/lukD 1 7 1 2 999 6 1 Saitama Bovine milk
12 1369 t160 VII 676D R1 hlg, lukE/lukD 1 7 1 2 1 6 1 Saitama Bovine milk
15 1 t1775 VII 514B Q7 hlg, lukE/lukD, pvl 1 999 3 1 2 9 1 Tochigi Bovine milk
15 81 t127 VII 514B Q3 hlg, lukE/lukD 1 999 2 0 2 6 1 Ishikawa Bovine milk
15 81 t127 VII 514B Q8 hlg, lukE/lukD 1 999 3 1 1 6 1 Iwate Bovine milk
15 81 t127 VII 595F Q6 hlg, lukE/lukD, pvl 1 999 2 0 1 6 1 Hokkaido Bovine milk
15 81 t127 VII 595F Q1, Q5 hlg, lukE/lukD 1 999 2 1 1 6 2 Iwate, Gunma Bovine milk
15 81 t127 VII 595F Q2 hlg, lukE/lukD 1 999 2 1 2 6 1 Kochi Bovine milk
15 81 t127 VII 595F Q4 hlg, lukE/lukD 1 999 3 1 1 6 1 Fukuoka Bovine milk
15 81 t127 VII 595F Q9 hlg, lukE/lukD 1 999 3 1 2 6 1 Nara Bovine milk
15 188 t189 V 676A K6 hlg, lukE/lukD 1 999 2 1 4 5 1 Tochigi Bovine milk
15 188 t189 V 676E K11 hlg, lukE/lukD 1 999 1 2 4 5 1 Hokkaido Bovine milk
15 188 t189 V 676E K1, K2, K4, K5, K7, K8, K9, K10, K12, K13 hlg, lukE/lukD 1 999 3 2 4 5 8 Hokkaido, Iwate, Saitama, Shizuoka, Gifu, Fukuoka Bovine milk
15 188 t1858 V 676E K3 hlg, lukE/lukD 1 999 3 1 4 6 1 Tottori Bovine milk
20 20 t164 VIII 595E U1, U4, U7 hlg, lukE/lukD 3 10 2 1 4 7 3 Iwate Bovine milk
20 20 t164 VIII 595E U11 hlg 3 10 2 1 4 7 1 Hiroshima Bovine milk
20 20 t164 VIII 595E U5 hlg, lukE/lukD 3 11 2 0 4 7 1 Gunma Bovine milk
20 20 t164 VIII 595E U10 hlg 3 11 2 1 4 7 1 Tokyo Bovine milk
20 20 t693 VIII 595E U9 hlg, lukE/lukD 3 10 2 1 4 0 1 Tokyo Bovine milk
20 20 t2109 VIII 595E U15 hlg, lukE/lukD 3 10 2 1 4 3 1 Ishikawa Bovine milk
20 20 t3277 VIII 595E U2, U18 hlg, lukE/lukD 3 10 2 1 4 6 2 Hokkaido, Ishikawa Bovine milk
20 20 t3332 VIII 595E U3 hlg, lukE/lukD 3 10 2 1 4 5 1 Hokkaido Bovine milk
20 20 t3929 VIII 514B U13, U14 hlg, lukE/lukD 3 8 2 1 4 3 1 Hyogo Bovine milk
20 20 t4542 VIII 595E U8 hlg, lukE/lukD 3 10 2 1 4 3 1 Saitama Bovine milk
20 20 t5267 VIII 595E U6 hlg, lukE/lukD 3 10 2 1 4 10 1 Niigata Bovine milk
20 20 t5412 VIII 595E U12 hlg, lukE/lukD 3 10 2 1 4 11 1 Hokkaido Bovine milk
20 1368 t3277 VIII 595E U17 hlg, lukE/lukD 3 10 2 1 4 6 1 Hyogo Bovine milk
20 1370 t881 VIII 595E U16 hlg, lukE/lukD 3 10 2 0 4 6 1 Ishikawa Bovine milk
25 25 t078 II 595A V5 hlg, lukE/lukD 3 5 3 2 4 8 1 Hokkaido Bovine milk
25 25 t258 II 514B V4 hlg, lukE/lukD 3 5 3 2 3 9 1 Fukuoka Bovine milk
25 26 t287 II 595A V1, V2 hlg, lukE/lukD 3 5 2 2 4 2 1 Tokyo Bovine milk
25 1372 t258 II 514B V3 hlg, lukE/lukD 3 3 3 2 3 9 1 Gunma Bovine milk
30 243 t021 IV 676C J hlg, pvl 2 3 2 2 2 8 1 Hokkaido Bovine milk
45 508 t050 VII 676G T1, T3 hlg 999 999 2 0 1 9 2 Gunma, Fukuoka Bovine milk
45 508 t362 VII 676G T4 hlg 999 999 2 0 1 1 1 Kochi Bovine milk
45 508 t630 VII 676G T2 hlg 999 999 2 0 1 8 1 Fukuoka Bovine milk
59 59 t216 VII 757E S hlg 2 999 4 1 4 7 1 Iwate Bovine milk
88 88 t1028 III 757A F3, F4 hlg, lukE/lukD 2 999 3 2 0 3 1 Shizuoka Bovine milk
88 88 t1028 III 757A F5 hlg, lukE/lukD 2 999 3 3 0 3 1 Shizuoka Bovine milk
88 88 t5264 III 757A F1 hlg, lukE/lukD 1 999 3 2 2 7 1 Ishikawa Bovine milk
88 1360 t729 III 757A F2 hlg, lukE/lukD 2 999 3 1 2 9 1 Hokkaido Bovine milk
97 97 t224 VI 838A O43 hlg, lukE/lukD, lukM/lukF′-PV 3 999 4 3 2 7 1 Hyogo Bovine milk
97 97 t224 VI 838A O56 hlg, lukE/lukD 3 999 4 3 2 7 1 Hyogo Bovine milk
97 97 t267 VI 838A O58 hlg, lukE/lukD 4 999 1 3 2 9 1 Hyogo Bovine milk
97 97 t267 VI 838A O38 hlg, lukE/lukD 4 999 2 2 2 9 1 Okinawa Bovine milk
97 97 t359 VI 838A O57 hlg, lukE/lukD 4 999 1 3 2 8 1 Hyogo Bovine milk
97 97 t1109 VI 838A O47, O48 hlg, lukE/lukD 4 999 3 3 2 2 1 Saitama Bovine milk
97 97 t1234 VI 838A O42 hlg, lukE/lukD 4 999 2 3 2 8 1 Gunma Bovine milk
97 124 t224 VI 838A O46 hlg, lukE/lukD 4 999 2 5 1 7 1 Hyogo Bovine milk
97 124 t189 VI 838A L hlg, lukE/lukD 4 999 3 5 999 5 1 Tochigi Bovine milk
97 124 t224 VI 838A O55 hlg, lukE/lukD 3 999 3 4 1 7 1 Gunma Bovine milk
97 124 t224 VI 838A O53 hlg, lukE/lukD 4 999 1 3 1 7 1 Nara Bovine milk
97 124 t458 VI 838A O50 hlg, lukE/lukD 4 999 3 4 999 1 1 Nara Bovine milk
97 124 t458 VI 838A O51, O52 hlg, lukE/lukD 4 999 3 4 1 1 1 Niigata Bovine milk
97 124 t2453 VI 838A P hlg, lukE/lukD 4 999 2 4 2 2 1 Tochigi Bovine milk
97 124 t5265 VI 838B O49 hlg, lukE/lukD 4 999 3 2 2 4 1 Tochigi Bovine milk
97 352 t203 VI 595E O23 hlg, lukE/lukD, lukM/lukF′-PV 4 999 4 3 2 7 1 Yamagata Bovine milk
97 352 t267 VI 352B O22 hlg, lukE/lukD, lukM/lukF′-PV 4 999 4 3 2 9 1 Hiroshima Bovine milk
97 352 t267 VI 595E O6 hlg, lukE/lukD, lukM/lukF′-PV 4 999 4 2 2 9 1 Hokkaido Bovine milk
97 352 t267 VI 595E O1, O4, O10, O11, O16, O17, O18, O32 hlg, lukE/lukD, lukM/lukF′-PV 4 999 4 3 2 9 6 Hokkaido, Tochigi, Chiba, Kyoto, Hyogo Bovine milk
97 352 t267 VI 757C O7 hlg, lukE/lukD, lukM/lukF′-PV 4 999 4 2 2 9 1 Ishikawa Bovine milk
97 352 t267 VI 757C O21 hlg, lukE/lukD, lukM/lukF′-PV 4 999 4 4 2 9 1 Hyogo Bovine milk
97 352 t267 VI 838A O45 hlg, lukE/lukD, lukM/lukF′-PV 999 999 4 2 2 9 1 Tottori Bovine milk
97 352 t267 VI 838A O41 hlg, lukE/lukD, lukM/lukF′-PV 4 999 2 2 2 9 1 Okinawa Bovine milk
97 352 t267 VI 838A O3 hlg, lukE/lukD 4 999 3 3 1 9 1 Hyogo Bovine milk
97 352 t267 VI 838A O36, O39, O40, O44 hlg, lukE/lukD, lukM/lukF′-PV 4 999 4 2 2 9 3 Fukuoka, Okinawa Bovine milk
97 352 t267 VI 838A O2 hlg, lukE/lukD 4 999 4 3 2 9 1 Hokkaido Bovine milk
97 352 t267 VI 838A O9, O12, O13, O31, O34, O35 hlg, lukE/lukD, lukM/lukF′-PV 4 999 4 3 2 9 5 Tokyo, Hyogo, Hiroshima, Kochi Bovine milk
97 352 t359 VI 838A O24 hlg, lukE/lukD, lukM/lukF′-PV 4 999 3 2 2 8 1 Hokkaido Bovine milk
97 352 t359 VI 838A O5, O20, O30 hlg, lukE/lukD, lukM/lukF′-PV 4 999 4 3 2 8 3 Tochigi, Hyogo, Kochi Bovine milk
97 352 t521 VI 595E O19 hlg, lukE/lukD, lukM/lukF′-PV 4 999 4 3 2 10 1 Hyogo Bovine milk
97 352 t1201 VI 838A O15 hlg, lukE/lukD, lukM/lukF′-PV 4 999 2 2 2 5 1 Hyogo Bovine milk
97 352 t1201 VI 838A O28, O29 hlg, lukE/lukD, lukM/lukF′-PV 4 999 3 2 2 5 2 Tochigi, Hyogo Bovine milk
97 352 t1201 VI 676F O26 hlg, lukE/lukD, lukM/lukF′-PV 4 999 3 2 2 5 1 Niigata Bovine milk
97 352 t1201 VI 757D O27 hlg, lukE/lukD, lukM/lukF′-PV 4 999 3 2 2 5 1 Ishikawa Bovine milk
97 352 t2844 VI 838A O25 hlg, lukE/lukD, lukM/lukF′-PV 3 999 3 2 2 4 1 Tokyo Bovine milk
97 352 t2844 VI 838A O8 hlg, lukE/lukD, lukM/lukF′-PV 4 999 3 2 2 4 1 Chiba Bovine milk
97 352 t3782 VI 838A O37 hlg, lukE/lukD, lukM/lukF′-PV 4 999 3 2 2 4 1 Okinawa Bovine milk
97 352 t5352 VI 838A O14 hlg, lukE/lukD, lukM/lukF′-PV 4 999 4 3 2 7 1 Gunma Bovine milk
97 1366 t1109 VI 838A O33 hlg, lukE/lukD, lukM/lukF′-PV 4 999 4 3 2 2 1 Niigata Bovine milk
97 1367 t521 VI 838A O54 hlg, lukE/lukD 4 999 3 2 2 10 1 Tochigi Bovine milk
509 89 t375 I 676A A3 none 2 999 1 1 1 7 IIIa 1 Tokyo Human wound
509 89 t375 I 676A A4, A6 hlg 2 999 1 1 1 7 IVE 2 Ibaraki Human phlegm, human synovial fluid
509 89 t375 I 676A A5, A7, A8 hlg 2 999 1 1 1 7 Untypeable 3 Ibaraki, Gunma Human eye mucus, human synovial fluid
509 89 t5266 I 676A A1 hlg, sagB 2 999 1 0 1 6 IIIa 1 Unknown Bovine milk
509 1373 t5262 I 676A A2 hlg 2 999 1 1 1 7 Untypeable 1 Ibaraki Human pus
705 705 t529 VI 595D N33 hlg, lukE/lukD, lukM/lukF′-PV, sagB, sagD 1 999 999 999 999 1 1 Chiba Bovine milk
705 705 t529 VI 595D N5, N11, N12 hlg, lukE/lukD, lukM/lukF′-PV, sagB, sagD 1 999 999 999 0 1 3 Gunma, Ishikawa, Okinawa Bovine milk
705 705 t529 VI 595D N1, N2, N4, N7, N9, N10, N13, N14, N15, N16, N17, N18, N20, N22, N24, N27, N28, N29, N30, N31, N32, N34, N36 hlg, lukE/lukD, lukM/lukF′-PV, sagB, sagD 1 999 2 999 0 1 23 Hokkaido, Tochigi, Saitama, Gunma, Ishikawa, Kochi, Hiroshima, Fukuoka, Okinawa Bovine milk
705 705 t529 VI 595D N3, N8, N19, N37, N38 hlg, lukE/lukD, lukM/lukF′-PV, sagB, sagD 1 999 2 999 999 1 5 Tokyo, Ishikawa, Gifu, Saga Bovine milk
705 705 t529 VI 595D N25 hlg, lukE/lukD, lukM/lukF′-PV, sagB, sagD 1 999 3 2 0 1 1 Hokkaido Bovine milk
705 705 t529 VI 595D N6 hlg, lukE/lukD, lukM/lukF′-PV, sagB, sagD 1 999 4 999 0 1 1 Ishikawa Bovine milk
705 1363 t529 VI 595D N21, N23 hlg, lukE/lukD, lukM/lukF′-PV, sagB, sagD 1 999 999 999 0 1 2 Tochigi, Okinawa Bovine milk
705 1364 t529 VI 595D N26 hlg, lukE/lukD, lukM/lukF′-PV, sagB, sagD 1 999 999 999 0 1 1 Tochigi Bovine milk
705 1365 t529 VI 595D N35 hlg, lukE/lukD, lukM/lukF′-PV, sagB, sagD 1 999 999 999 999 1 1 Hokkaido Bovine milk
1359 t5263 II 919A C hlg, sagB 3 999 1 2 3 8 1 Hyogo Bovine milk
1361 t5260 Untypeable 919B I hlg, sagB, sagD 999 999 3 999 3 7 1 Iwate Bovine milk
1371 t5261 IV 1000A W hlg 3 999 3 999 3 8 1 Hiroshima Bovine milk
a

MRSA isolates are highlighted in boldface. The major bovine lineages were CC97 and CC705.

b

“999” indicates that there was no amplification of SIRUs.

c

Locations were dairy farms or hospitals.