TABLE 2.
Assay specificity and sensitivitya
| Viral pathogen(s) | TSE signal/lowest detected TCID50 for HAdV serotype: |
Luminex RVP assay callb | ||||
|---|---|---|---|---|---|---|
| 3 | 4 | 7 | 14 | 21 | ||
| HAdV-3 | +/5 × 103 | − | − | − | − | Adenovirus |
| HAdV-4 | − | +/9.5 × 103 | − | − | − | Adenovirus |
| HAdV-7 | − | − | +/1.1 × 104 | − | − | Adenovirus |
| HAdV-7a | − | − | +/ND | − | − | Adenovirus |
| HAdV-14 | − | − | − | +/5 × 103 | − | Adenovirus |
| HAdV-21 | − | − | − | − | +/4.6 × 105 | Adenovirus |
| HAdVs 3 and 7 | + | − | + | − | − | NA |
| HAdVs 4, 14, and 21 | − | + | − | + | + | NA |
| HAdVs 3, 4, 7, 14, and 21 | + | + | + | + | + | NA |
| HAdVs 1, 2, 5, 6, 9, 11, 16, 17, 31, 34, 35, and 40 | − | − | − | − | − | ND |
| Influenza A virus H1N1 | − | − | − | − | − | Influenza A virus H1 |
| Influenza A virus H3N2 | − | − | − | − | − | Influenza A virus H3 |
| Influenza B virus | − | − | − | − | − | Influenza B virus |
| Coronavirus OC43 | − | − | − | − | − | Coronavirus OC43 |
| Coronavirus SARS | − | − | − | − | − | Coronavirus SARS |
| Parainfluenza virus 2 | − | − | − | − | − | Parainfluenza virus 2 |
| Parainfluenza virus 3 | − | − | − | − | − | Parainfluenza virus 3 |
| RSV A | − | − | − | − | − | RSV A |
| RSV B | − | − | − | − | − | RSV B |
| Metapneumovirus | − | − | − | − | − | Metapneumovirus |
| Enterovirus | − | − | − | − | − | Entero-/rhinovirus |
| Rhinovirus | − | − | − | − | − | Entero-/rhinovirus |
| Blank | − | − | − | − | − | − |
a
Assay specificity was assessed by testing with the pathogens indicated. Assay sensitivity was assessed by determining the lowest dilution detected from serial dilutions for each reference HAdV strain tested (see the text for details). The corresponding TCID50 of the lowest dilution detected is indicated. The coinfections were not tested by a commercial method because they do not distinguish serotypes.
b
NA, not available. ND, samples were not tested with the Luminex RVP kit but rather with the Argene adenovirus r-gene kit.