TABLE 3.
Comparison of PCR/microneutralization and Luminex HAdV assay results obtained at NHRCa
| HAdV serotype | No. of samples with indicated results in the two assays |
Sensitivity (%) | Specificity (%) | |||
|---|---|---|---|---|---|---|
| W+ N+ | W− N+ | W+ N− | W− N− | |||
| 3 | 9 | 1 | 0 | 94 | 90 | 100 |
| 4 | 19 | 0 | 2b,c | 83 | 100 | 98 |
| 7 | 10b | 0 | 0 | 94 | 100 | 100 |
| 14 | 19c | 0 | 0 | 85 | 100 | 100 |
| 21 | 17 | 3 | 0 | 84 | 85 | 100 |
a
Comparison of the results obtained by PCR/microneutralization and with the Luminex HAdV assay. W, result (+ or −) by the multiplexed Luminex HAdV assay; N, result (+ or −) by PCR/microneutralization. Sensitivity and specificity were based on true positives and true negative, defined as specimens giving positive or negative results according to the PCR/microneutralization assay. The sensitivity and specificity were calculated according to the methods reported in Cumitech 31 (9).
b
One HAdV-7 sample was positive for both HAdV-4 and HAdV-7.
c
One HAdV-14 sample was positive for both HAdV-14 and HAdV-4.