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Specificity of LAMP assay. Lane 1, 100-bp DNA ladder; lane 2, negative control; lanes 3 and 4, CMV, for which LAMP assay products were obtained; lanes 5, 6, 7, 8, and 9, other organisms (HSV-1, VZV, adenovirus, Aspergillus flavus, and Staphylococcus aureus, respectively), for which no LAMP products were obtained. The products amplified by the LAMP assay exhibit a typical ladder-like pattern on electrophoresis, indicating the formation of stem-loop DNA with inverted repeats.
