TABLE 3.

Evaluation of the qPCR assay using 500 clinical specimens^a

Serovar	No. (%) of predicted matches
	DNA sequencing	qPCR
		CT group	CT serovar
A	0	0	0
B/Ba	3	3	3
C	4	4	4
D	163	150	143
E	48	47	46
F	19	18	16
G	154	140	137
H	4	4	4
I	1	0	0
J	76	70	67
K	4	3	3
L1	0	0	0
L2	0	0	0
L3	0	0	0
Subtotal	476 (95.2)	439 (92.2)	423 (88.9)
CT-POSb	11	10	6
CT-NEGc	13	6	4
Total	500	455 (91.0)	433 (86.6)

^aEvaluation of the qPCR algorithm for C. trachomatis serovar prediction using clinical specimens. DNA sequencing was used as the gold standard for typing, to which the PCR results were compared. Anogenital specimens (n = 494) and ocular specimens (n = 6) were used for this comparison. Specimens were identified by qPCR as matching the CT group (B, C, or I group) and/or the CT serovar, as determined by sequencing.

^bEleven specimens tested positive for C. trachomatis by sequencing (CT-POS) without serovar identification. Ten of these were positive by qPCR group-specific PCR.

^cThirteen specimens tested negative for C. trachomatis by sequencing (CT-NEG). Six of these were positive by qPCR group-specific PCR.