TABLE 1.
Performance of the new RATa compared to real-time RT-PCR for the detection of pandemic influenza A/H1N1 2009 virus
| Detection component of the new RAT | No. of cases |
% Sensitivity (95% CIf) | % Specificity (95% CI) | PPVb (%) | NPVc (%) | ||||
|---|---|---|---|---|---|---|---|---|---|
| Total | PCR+ RAT+ | PCR+ RAT− | PCR− RAT+ | PCR− RAT− | |||||
| Detection component for 2009 H1N1 | |||||||||
| Paired samplesd | 561 | 186 | 55 | 0 | 320 | 77 (72-82) | 100 (99-100) | 100 | 85 |
| Unpaired samplee | 198 | 55 | 17 | 1 | 125 | 76 (65-84) | 99 (96-100) | 98 | 88 |
| Overall | 759 | 241 | 72 | 1 | 445 | 77 (72-81) | 100 (99-100) | 100 | 86 |
| Detection component for influenza A virus | |||||||||
| Paired samples | 561 | 152 | 89 | 1 | 319 | 63 (57-69) | 100 (98-100) | 99 | 78 |
| Unpaired sample | 198 | 42 | 30 | 0 | 126 | 58 (47-69) | 100 (97-100) | 100 | 81 |
| Overall | 759 | 194 | 119 | 1 | 445 | 62 (57-67) | 100 (99-100) | 100 | 79 |
a
As determined by SD Bioline Influenza Ag A/B/A(H1N1) Pandemic.
b
PPV, positive predictive value.
c
NPV, negative predictive value.
d
Two nasopharyngeal swabs (placed in 2 test tubes—one containing buffer solution and one containing viral transport medium) from the same patient at the same time were prepared for RAT and real-time RT-PCR.
e
One nasopharyngeal swab (placed in a tube containing viral transport medium) was used for RAT and real-time RT-PCR.
f
CI, confidence interval.