TABLE 1.
Performance of shortened E. coli/P. aeruginosa PNA FISH versus routine laboratory identification methods and standard PNA FISH
| Study site | Sensitivity for E. coli |
Sensitivity for P. aeruginosa |
Specificity |
Blood culture system used | |||
|---|---|---|---|---|---|---|---|
| Routine | Stnda | Routine | Stnd | Routine | Stnd | ||
| A | 51/51 | 51/51 | 12/12 | 12/12 | 54/54 | 54/54 | Bactec |
| B | 51/51 | 51/51 | 9/9 | 9/9 | 40/40 | 40/40 | BacT/Alert |
| C | 17/17 | 17/17 | 7/7 | 7/7 | 51/51 | 51/51 | Bactec |
| D | 32/32 | 32/32 | 7/8b | 7/7 | 36/36 | 37/37 | Bactec |
| Total (n = 368) | 100% (151/151) | 100% (151/151) | 97.2% (35/36) | 100% (35/35) | 100% (181/181) | 100% (182/182) | |
| 95% CI | 98.0-100 | 98.0-100 | 85.5-99.9 | 91.8-100 | 98.4-100 | 98.4-100 | |
a
Stnd, standard PNA FISH procedure.
b
One sample was identified as a mixed P. aeruginosa/VRE culture by routine methods and was negative for both the rapid and standard procedures.