TABLE 1.
Primers used in PCR amplification
| Target gene(s) | Name | DNA sequence (5′ to 3′) | Tm (°C) | Expected amplicon size (bp) | GenBank accession. no. | Reference |
|---|---|---|---|---|---|---|
| blaSHV | OS5 | TTATCTCCCTGTTAGCCACC | 60 | 796 | GQ463148 | 1 |
| OS6 | GATTTGCTGATTTCGCTCGG | |||||
| blaOXA | OO-1 | ACCAGATTCAACTTTCAA | 55 | 589 | GQ896560 | 22 |
| OO-2 | TCTTGGCTTTTATGCTTG | |||||
| blaTEM | TemA | ATAAAATTCTTGAAGAC | 50 | 1,073 | AY463797 | 11 |
| TemB | TTACCAATGCTTAATCA | |||||
| blaPSE | pse1-F | CGCTTCCCGTTAACAAGTAC | 60 | 419 | M69058 | 23 |
| pse1-R | CTGGTTCATTTCAGATAGCG | |||||
| strA-strB | SAF | AGCAGAGCGCGCCTTCGCTG | 62 | 703 | NC_001740 | 12 |
| SBR | CCAAAGCCCACTTCACCGAC | |||||
| aadA2 | AadA2F | TGTTGGTTACTGTGGCCGTA | 60 | 622 | AF071555 | 35 |
| AadA2R | GATCTCGCCTTTCACAAAGC | |||||
| sul1 | Sul1F | CTTCGATGAGAGCCGGCGGC | 65 | 436 | X12869 | 23 |
| Sul1b | GCAAGGCGGAAACCCGCGCC | |||||
| sul2 | Sul2F | TCAACATAACCTCGGACAGT | 60 | 707 | M36657 | 23 |
| Sul2R | GATGAAGTCAGCTCCACCT | |||||
| tet(A) | TetAF | GTAATTCTGAGCACTGTCGC | 60 | 956 | X61367 | 12 |
| TetAR | CTGCCTGGACAACATTGCTT | |||||
| tet(B) | TetBF | ACGTTACTCGATGCCAT | 50 | 1,169 | AM412236 | 12 |
| TetBR | AGCACTTGTCTCCTGTT | |||||
| tet(C) | TetCF | AACAATGCGCTCATCGT | 52 | 1,138 | Y19114 | 17 |
| TetCR | GGAGGCAGACAAGGTAT | |||||
| tet(G) | TetGF | CCGGTCTTATGGGTGCTCTA | 56 | 603 | F071555 | 35 |
| TetGR | CCAGAAGAACGAAGCCAGTC | |||||
| Integron variable region | 5′CS | GGCATCCAAGCAGCAAG | 56 | Variable | M73819 | 12 |
| 3′CS | AAGCAGACTTGACCTGA | |||||
| Integrase | Int1F | GCCTTGCTGTTCTTCTAC | 55 | 558 | X12870 | 22 |
| Int1B | GATGCCTGCTTGTTCTAC | |||||
| 16S rRNA gene | 16SF | TGTTGTGGTTAATAACCGCA | 56 | 571 | EU073022 | 44 |
| 16SR | CACAAATCCATCTCTGGA | |||||
| tet(B)a | TetB2 | CCAGTAGCTCCTGTGAT | 50 | 282 | AM412236 | This study |
| blaTEMa | TemC | AGCATCTTTTACTTTCA | 52 | 300 | AY463797 | This study |
a
Primers were used together with primers TetBF and TemA, respectively, only to produce probes.