TABLE 1.

Results of histopathology, PCR (DNA extracted with Takara Dexpat), and DNA sequence analysis of 51 FFPE human tissues

Sample	Tissue site (type of specimen)	Clinical diagnosis	Histopathology (GMS)a	PCR resultsb		Result of sequencing
				HKG	ITS3-4
1	Lung	Aspergillosis	Sparse	+	+	Aspergillus flavus
2	Lung	Blastomycosis	Abundant	+	+	B. dermatitidis
3	Sinus	Aspergillosis	Sparse	−	−
4	Prostate	Aspergillosis	Medium	+	+	A. flavus
5	Unknown	Zygomycosis/Aspergillosis	Sparse	+	+	Aspergillus niger
6	Nasal cartilage	Zygomycosis	Sparse	−	−
7	Sinus	Aspergillosis	Medium	+	+	Aspergillus fumigatus
8	Sinus	Aspergillosis	Abundant	+	+	A. fumigatus
9	Nasal septum	Aspergillosis	Abundant	−	−
10	Orbital tissue	Aspergillosis	Abundant	+	+	A. niger
11	Turbinate	Aspergillosis	Abundant	+	+	A. flavus
12	Sinus	Aspergillosis	Sparse	−	−
13	Sinus	Aspergillosis	Abundant	+	+	A. flavus
14	Colon	Histoplasmosis	Abundant	+	+	H. capsulatum
15	Unknown	Aspergillosis	Abundant	+	+	A. flavus
16	Unknown	Aspergillosis	Abundant	+	+	A. flavus
17	Lung	Blastomycosis	Abundant	+	+	B. dermatitidis
18	Bone marrow	Histoplasmosis	Abundant	+	+	H. capsulatum
19	Bone marrow	Histoplasmosis	Abundant	+	+	H. capsulatum
20	Bone marrow	Histoplasmosis	Abundant	+	+	H. capsulatum
21	Bone marrow	Histoplasmosis	Abundant	+	+	H. capsulatum
22	Bone marrow	Histoplasmosis	Abundant	+	+	H. capsulatum
23	Lung	Aspergillosis	Sparse	−	−
24	Cardiac muscle	Zygomycosis	Sparse	−	−
25	Nasal	Aspergillosis	Medium	+	+	A. flavus
26	Skin	Zygomycosis	Abundant	+	+	Apophysomyces elegans
27	Colon	Aspergillosis	Abundant	+	+	A. fumigatus
28	Colon	Aspergillosis	Abundant	+	+	A. fumigatus
29	Lung	Histoplasmosis	Abundant	+	+	H. capsulatum
30	Skin	Zygomycosis	Medium	+	−
31	Lung	Zygomycosis	Abundant	+	+	Rhizopus oryzae/Amylomyces rouxii
32	Lung	Zygomycosis	Sparse	+	−
33	Kidney	Zygomycosis	Abundant	+	+	Rhizopus oryzae/A. rouxii
34	Lung	Zygomycosis	Sparse	+	+	R. oryzae/R. microsporus/R. azygosporus
35	Lung	Aspergillosis	Sparse	+	−
36	Lung	Aspergillosis	Sparse	+	−
37	Kidney	Aspergillosis	Medium	+	−
38	Unknown	Zygomycosis	Sparse	+	+	R. oryzae/A. rouxii
39	Unknown	Zygomycosis	Sparse	+	+	R. oryzae/A. rouxii
40	Unknown	Zygomycosis	Sparse	+	+	Saksenaea vasiformis
41	Nasal	Zygomycosis	Medium	+	+	C. echinulata/C. septata
42	Forearm	Zygomycosis.	Medium	+	+	R. oryzae/R. azygosporus/R. microsporus
43	Lung	Zygomycosis	Sparse	+	+	R. microsporus/R. azygosporus
44	Abdominal	Zygomycosis	Medium	+	+	R. oryzae/A. rouxii
45	Lung	Zygomycosis	Sparse	+	−
46	Skin	Zygomycosis	Abundant	+	+	R. microsporus/R. azygosporus
47	Spleen	Zygomycosis	Medium	+	+	R.s oryzae/A. rouxii
48	Skin	Zygomycosis	Medium	+	+	R. oryzae/A. rouxii
49	Axilla	Zygomycosis	Medium	+	+	R. oryzae/A. rouxii
50	Eye	Histoplasmosis	Abundant	+	+	H. capsulatum
51	Lung	Aspergillosis	Medium	+	+	A. fumigatus

^aFungal structures are reported as abundant, medium and sparse.

^bHKG, housekeeping gene (human β-globin gene). +, positive; −, negative.