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Molecular characterization of 15 single colonies isolated from the original M. avium-M. kansasii mixed culture by PRA-ITS (A), PCR-IS1245 (B), RFLP-IS1245 (C), and pulsed-field gel electrophoresis (D). Lanes: colonies 88.1 to 88.4, M. avium; colonies 88.5 to 88.15, M. kansasii; −, PCR negative control (water); +, PCR positive control (DNA from M. avium ATCC 25291T). Asterisks indicate RFLP-IS1245 band polymorphisms in M. avium colonies. On the right are molecular size markers.
