Table 1.
Classification of phytoplasmas in the peach X-disease phytoplasma group (16SrIII) based on RFLP patterns derived from 16S rRNA gene F2nR2 sequences
Novel subgroups delineated in the present study are highlighted in bold. Asterisks indicate variants of the designated subgroup or pattern type. In the Subgroup column, letters in parentheses denote the RFLP pattern types of rrnA/rrnB. Representative (or reference) strains are the first strains for which a given subgroup pattern type was recognized.
| Subgroup | Virtual pattern | GenBank accession no. | Representative strain | Original source | Notes |
|---|---|---|---|---|---|
| 16SrIII-A | 16SrIII-A | L33733 | Peach X-disease phytoplasma CX | Peach (Canada) | No heterogeneous rrn reported so far |
| 16SrIII-B | 16SrIII-B | AF175304 | Clover yellow edge phytoplasma CYE-C | Clover (Canada) | No heterogeneous rrn reported so far |
| 16SrIII-C | 16SrIII-C | FJ376626 | Pecan bunch phytoplasma PB1 | Pecan (Georgia, USA) | No heterogeneous rrn reported so far |
| 16SrIII-D | 16SrIII-D | FJ376627 | Goldenrod yellows phytoplasma GR1 | Goldenrod (New York, USA) | No heterogeneous rrn reported so far |
| 16SrIII-(A*/?)E | 16SrIII-A*, rrnA | AF190228 | Spiraea stunt phytoplasma SP1 | Spiraea (New York, USA) | Incomplete; rrnB sequence unavailable |
| 16SrIII-F | 16SrIII-F | AF510724 | Milkweed yellows phytoplasma MW1 | Milkweed (New York, USA) | No heterogeneous rrn reported so far |
| 16SrIII-(G/A*)G | 16SrIII-G, rrnA | AF190226 | Walnut witches' broom phytoplasma WWB | Walnut (Georgia, USA) | Composite pattern is available (Fig. 4) |
| 16SrIII-A*, rrnB | AF190227 | Walnut witches' broom phytoplasma WWB | Walnut (Georgia, USA) | Composite pattern is available (Fig. 4) | |
| 16SrIII-(A*/?)H | 16SrIII-A*, rrnA | AF190223 | Poinsettia branch-inducing phytoplasma PoiBI | Poinsettia (USA) | Incomplete; rrnB sequence unavailable |
| 16SrIII-(A*/?)I | 16SrIII-A*, rrnA | AF060875 | Virginia grapevine yellows phytoplasma VGYIII | Grapevine (Virginia, USA) | Incomplete; rrnB sequence unavailable |
| 16SrIII-J | 16SrIII-J | AF147706 | Chayote witches' broom phytoplasma ChWBIII (Ch10) | Chayote (Brazil) | No heterogeneous rrn reported so far |
| 16SrIII-K | 16SrIII-K | AF274876 | Strawberry leafy fruit phytoplasma SLF | Strawberry (Maryland, USA) | No heterogeneous rrn reported so far |
| 16SrIII-L | 16SrIII-L | EU169138 | Poinsettia exuberant flower-inducing phytoplasma EF-MM | Poinsettia (Mexico) | No heterogeneous rrn reported so far |
| 16SrIII-M | 16SrIII-M | FJ226074 | Montana potato purple top phytoplasma PPT-MT117-1 | Potato (Montana, USA) | No heterogeneous rrn reported so far |
| 16SrIII-N | 16SrIII-N | FJ376629 | Alaska potato purple top phytoplasma PPT-AK6 | Potato (Alaska, USA) | No heterogeneous rrn reported so far |
| 16SrIII-(P/O)P | 16SrIII-O, rrnB | AF370120 | Dandelion virescence phytoplasma DanVir | Dandelion (Lithuania) | Composite pattern is available (Fig. 4) |
| 16SrIII-P, rrnA | AF370119 | Dandelion virescence phytoplasma DanVir | Dandelion (Lithuania) | Composite pattern is available (Fig. 4) | |
| 16SrIII-Q | 16SrIII-Q | AF302841 | Black raspberry witches'-broom phytoplasma BRWB | Black raspberry (Oregon, USA) | No heterogeneous rrn reported so far |
| 16SrIII-(R/B)R | 16SrIII-R, rrnA | AF373105 | Cirsium white leaf phytoplasma CirWL | Cirsium (Lithuania) | Composite pattern is available (Fig. 4) |
| 16SrIII-B, rrnB | AF373106 | Cirsium white leaf phytoplasma CirWL | Cirsium (Lithuania) | Composite pattern is available (Fig. 4) | |
| 16SrIII-S | 16SrIII-S | L04682 | Western peach X-disease phytoplasma WX | Peach (California, USA) | No heterogeneous rrn reported so far |