Table 2.

PCR-based detection of L. pneumophila

Templates included clinical lung autopsy material and tissue culture spiked with live bacterial culture (prepared using a Qiagen tissue extraction kit) or pure bacterial culture (prepared from boiled cell lysates).

Sample*	Patient	Description	dnaX	ftsZ	trpS	mip	16S†	β-globin‡
L. pneumophila ATCC 33153	na	Pure culture	+	+	+	+	+	−
L. micdadei ATCC 33218	na	Pure culture	−	−	−	−	+	−
293T tissue culture	na	Tissue culture	−	−	−	−	−	+
293T+L. pneumophila	na	Spiked tissue	+	+	+	+	+	+
293T+L. micdadei	na	Spiked tissue	−	−	−	−	+	+
05-L-011	1	Autopsy tissue	+	+	+	+	+	+
05-L-012 (DFA+)	1	Autopsy tissue	+	+	+	+	+	+
05-L-013	1	Autopsy tissue	+	+	+	+	+	+
05-L-014	2	Autopsy tissue	+	+	+	+	+	+
05-L-015	2	Autopsy tissue	+	+	+	+	+	+
05-L-024 (DFA+)	2	Autopsy tissue	+	+	+	+	+	+
05-L-016	3	Autopsy tissue	+	+	+	+	+	+
05-L-017	3	Autopsy tissue	+	+	+	+	+	+
05-L-025 (DFA+)	3	Autopsy tissue	−	−	−	−	−	−
05-L-018	4	Autopsy tissue	+	+	+	+	+	+
05-L-019	4	Autopsy tissue	+	+	+	+	+	+
05-L-027 (DFA+)	4	Autopsy tissue	+	+	+	+	+	+
05-L-020	5	Autopsy tissue	+	+	+	+	+	+
05-L-021	5	Autopsy tissue	+	+	+	+	+	+
05-L-026 (DFA+)	5	Autopsy tissue	+	+	+	+	+	+
05-L-022	6	Autopsy tissue	+	+	+	+	+	+
05-L-023	6	Autopsy tissue	+	+	+	+	+	+
05-L-028 (DFA+)	6	Autopsy tissue	+	+	+	+	+	+
05-L-029 (DFA+)	7	Autopsy tissue	+	+	+	+	+	+

na, Not applicable.

*DFA+, DFA detected L. pneumophila serogroup 1 in the clinical specimen.

†Legionella genus-specific primers (Raggam et al., 2005).

‡Human-specific primers (Keller & Mannack, 1993).