Fig. 1.
Schematic representation (not to scale) showing the construction of recombinant intimin β and γ. The C-terminal regions including the receptor-binding regions of intimin α (eae280α), intimin β (eae280β) and intimin γ (eae280γ) are represented by different shading. The percentage amino acid identity of each region of intimin β and intimin γ compared to that of intimin α is indicated. The position of the restriction site, SalI, used to construct the recombinant intimin is given in nucleotides and the position corresponding to SalI in intimin is represented as a solid line.