Table 3.
Interaction of recombinant truncated PilQ proteins in band-shift analysis with ss- and dsDNA
ssDNA comprised primers T1, T2, T3, T7 and T8, and dsDNA comprised T1+T2, T3+T4 and T7+T8 hybrids (see Table 2). +, Positive reactivity in the band shift; (+), weak binding of DNA; −, no visible band shift.
| PilQ recombinant | DNA substrates | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| ssDNA | dsDNA | ||||||||
| T1 | T2 | T3 | T4 | T7 | T8 | T1+T2 hybrid | T3+T4 hybrid | T7+T8 hybrid | |
| DUS+ | DUS+ | DUS− | DUS− | DUS+ | DUS+ | DUS+ | DUS− | DUS+ | |
| PilQ25–132 | − | − | − | − | − | (+) | (+) | + | (+) |
| PilQ25–354 | + | + | + | + | + | + | + | + | + |
| PilQ218–478 | − | (+) | − | − | (+) | − | − | (+) | (+) |
| PilQ352–761 | − | − | − | − | − | − | − | − | − |
| OpcA* | − | − | − | − | − | − | − | − | − |
| HmbR* | − | − | − | − | − | − | − | − | − |
| MutY† | (+) | (+) | (+) | (+) | (+) | (+) | + | + | + |
*Negative control.
†Positive control.