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. 2007 Aug;88(Pt 8):2280–2290. doi: 10.1099/vir.0.82809-0

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Copyright © 2007, SGM

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Fig. 4. — Virion RNP core co-sedimentation assay. Aliquots of the indicated radiolabelled, in vitro-translated M1 polypeptides were incubated in the presence (+) or absence (−) of detergent-disrupted virus and analysed by SDS-PAGE and (a) Coomassie blue staining or (b) autoradiography before (T) or after separation into pellet (P) and supernatant (S) fractions by centrifugation. (a) Molecular mass markers are indicated on the left and the position of specified proteins on the right. (c) The percentage of M1 polypeptides in the pellet fraction was quantified by densitometry. The mean±range of two or three independent experiments is plotted.