Fig. 2.

Analysis of the effect of poxvirus infection on MNV replication. (a) BHK cells were mock-infected (M) or infected with either VACV (MVA-T7) or FWPV (FPV-T7) expressing T7 RNA polymerase and subsequently transfected with MNV VPg-linked RNA. Levels of the viral RNA-dependent RNA polymerase (NS7) and minor capsid protein (VP2) were analysed by Western blotting with rabbit polyclonal antisera. In parallel, the virus yield was determined and expressed as TCID₅₀ per 35 mm dish. Transfections were carried out in triplicate; error bars represent sd. (b) BHK cells were either mock-infected (M) or infected with MVA-T7 or FPV-T7 and subsequently transfected with a cDNA construct containing the entire MNV genome under the control of a T7 RNA polymerase promoter (pT7 : MNV-G). NS7 expression levels were subsequently analysed by Western blotting.