Fig. 2.
(a) ChIP analysis of MRC-5 cells, uninfected (Un) or latently infected with in1382 and, at 6 days p.i., either superinfected (+) or not (−) with wt HSV-2 strain 333 for 6 h in the presence of PAA. Antibodies used were anti-acetyl histone H4 (AcH4) or anti-trimethyl K9 histone H3 (mK9). PCRs were performed using primers specific for the HSV-1 ICP0 promoter. (b) Left panel: ChIP analysis of MRC-5 cells latently infected with in1382 and, at 6 days p.i., either superinfected (+) or not (−) with wt HSV-2 strain 333 for 6 h in the presence of PAA. Antibodies used were anti-acetyl histone H4 (AcH4), chemically acetylated H4 (AcH4a) or anti-acetyl histone H3 (acetyl K9 and K14) (AcH3). PCRs were performed using primer sets to the ICP0, LAT, gC or GAPDH promoters. Right panel: PCRs using ICP0 primers on input chromatin.