Table 1.

Effect of inhibitors divalent cations on the proteolytic activity of karilysin.

Inhibitors and metal ions	Concentration	% of control
Pefablock	5 mM	97
	10 mM	100
TLCK	50 μM	101
	100 μM	105
Leupeptin	50 μM	108
	100 μM	106
E-64	50 μM	104
	100 μM	102
Iodoacetic acid	5 mM	105
	10 mM	109
1,10-Phenanthroline	5 mM	3
	10 mM	0
EDTA	5 mM	2
	10 mM	0
CaCl2	2 mM	355
	5 mM	362
	10 mM	268
ZnCl2	2 mM	0
	5 mM	0
	10 mM	0

Activity determined at 37°C in 100mM Tris-HCl, pH 8.0, using FITC-casein as a substrate was taken as 100%.