Fig 4.
Oxidative stress caused by Dicer deficiency. (A) Two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis using whole brain extracts revealed that the proteins associated with vesicle transport, stress, redox, and actin cytoskeleton regulations are highly increased in the mutants. (B) H2O2 is increased in Dicer mutant brain. Data are expressed as means ± standard deviation. *p < 0.001. (C) Confirmation of upregulated stress and redox regulatory proteins, catalase and peroxiredoxin, in brains of adult mutant mice with severe phenotypes. (D) Immunohistochemical staining shows upregulated catalase in white matter tracts of cerebellum in mutant mice. Neuronal nuclei (NeuN) staining indicates grey/white matter boundaries in cerebellum. Scale bar represents 100μm. PLP = proteolipid protein; WT = wild-type; MT = mutant; GAPDH = glyceraldehyde phosphate dehydrogenase.