Fig. 7.

Intranasal infection with and without anaesthesia. (a) Mice were infected intranasally with M3-LUC (10⁴ p.f.u.), either awake or while anaesthetized, then imaged for luciferase expression every 3–5 days. Each point corresponds to one mouse. The horizontal dashed lines mark an arbitrary sensitivity threshold. (b) Representative images from the data summarized in (a). (c) At 1 month p.i., mice were analysed for viral genome loads in the spleen and SCLN by real-time PCR. The top panel shows the cellular control (APRT), the middle panel the MuHV-4 M2 gene, and the bottom panel the M2 load normalized by APRT. Each point corresponds to one mouse. There was no statistically significant difference between mice given anaesthesia (Inf. lung) or not (Inf. nose).