Fig. 3.

Latent DNA loads and explant reactivation capability of virus recombinants. (a, b, c) Latent DNA loads: real-time PCR was performed on DNA extracted from latently infected ganglia (CII, CIII and CIV pooled from five mice) using as targets ICP0 and APRT. Values represent the mean±sem of the numbers of the HSV genome copies per 10⁴ copies of APRT of three real-time PCRs. (d, e, f) Viral explants reactivation titres: CII, CIII and CIV ganglia from latently infected mice were explant cultured for 5 days and assayed for reactivated virus. Symbols represent viral titres detected per mouse and the bars are their mean.