Table 1.
Efficiency of virus production from 293T cells transfected with pgag-pol, pPH-gag-pol or pPH4A-gag-pol expression vector
| Experiment | Plasmid | p24CA (ng per well)* | Virus production efficiency (B/A) | Fold increase relative to pgag-pol | |
|---|---|---|---|---|---|
| Cell lysate (A) | Culture supernatant (B) | ||||
| 1 | pgag-pol | 4 043 | 4 869 | 1.204 | – |
| pPH-gag-pol | 1 989 | 8 363 | 4.206 | 3.49 | |
| pPH4A-gag-pol | 3 175 | 103 | 0.033 | 0.03 | |
| 2 | pgag-pol | 3 521 | 3 887 | 1.104 | – |
| pPH-gag-pol | 2 638 | 7 688 | 2.914 | 2.64 | |
| pPH4A-gag-pol | 5 913 | 1 125 | 0.190 | 0.17 | |
| 3 | pgag-pol | 3 359 | 4 160 | 1.239 | – |
| pPH-gag-pol | 1 454 | 5 172 | 3.558 | 2.87 | |
| pPH4A-gag-pol | 4 226 | 75 | 0.018 | 0.01 | |
| 4 | pgag-pol | 9 666 | 8 996 | 0.931 | – |
| pPH-gag-pol | 4 699 | 18 273 | 3.889 | 4.18 | |
| pPH4A-gag-pol | 5 527 | 534 | 0.097 | 0.10 | |
*Cells grown in six-well plates were transfected using Lipofectamine 2000 according to the manufacturer's protocol (Invitrogen).