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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Leuk Lymphoma. 2010 Jun;51(6):1098–1107. doi: 10.3109/10428191003754624

Figure 1. The chemotactic response of Ba/F3 cells to SDF1α is suppressed by p185Bcr-Abl transformation and pharmacological inhibition of PI3 kinases.

Figure 1

A. Transformation of Ba/F3 cell by p185Bcr-Abl impaired SDF1α-stimulated chemotaxis of Ba/F3 cell. The left panel represents one of nine transwell cell migration experiments. The vertical axis shows the percentage of migrated cell and is expressed as the average +/− S.D. of triplicate wells. The chemotactic index shown in the right panel is expressed in vertical axis as the average +/− S.D (*P < 0.05) calculated from 9 independent experiments. B. Inhibition of PI3K pathway impaired the SDF1α-stimulated chemotaxis of both Ba/F3 cell and p185wt cell. The Ba/F3 cells and p185wt cells were pre-incubated with or without 50µM LY294002, as indicated, for 5 hours. 1×105 cells were tested in trans-well migration assay at the presence or absence of SDF1α (100 ng/ml) in bottom chamber. The data represents one of three independent migration experiments. The percentage of migrated cells is shown in vertical axis as the average +/− S.D. calculated from triplicate wells.