Fig. 2.

Immune serum limits MuHV-4 lytic gene expression in lungs and noses. (a) BALB/c mice were infected i.n. with luciferase⁺ MuHV-4 (3×10⁴ p.f.u.) and at the same time given immune serum (200 μl), naive serum (200 μl) or nothing i.p. Virus replication was monitored by luciferin injection and CCD camera scanning at 5 days post-infection. (b) Quantification of signals shown in (a). Each point shows the luciferase signal of one mouse; × shows mean values. Immune serum reduced luciferase expression significantly compared with naive serum (P<0.02 for noses, P<0.004 for lungs) or no-serum controls (P<0.03 for noses, P<0.006 for lungs) using Student's two-tailed t-test. (c) Noses from (a) were titrated for infectious virus by plaque assay. Each point shows the titre for one mouse. Immune serum reduced titres significantly compared with naive serum (P<0.005). Titres with immune serum were also lower than with virus only, although the wide scatter of values made this difference non-significant by t-test (P=0.06).