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. 2009 Nov;90(Pt 11):2592–2603. doi: 10.1099/vir.0.014266-0

Fig. 4.

Fig. 4.

Reduction in in vivo MuHV-4 infectivity by glycoprotein-specific mAbs. (a) C57BL/6 mice were infected i.n. with MuHV-4 (3×104 p.f.u.) and at the same time given antibody i.p. (500 μg). Infectious virus titres in lungs were then determined by plaque assay. Each point shows the titre for one mouse; × shows mean values. The gp70-specific mAb LT-6E8 reduced virus titres significantly at day 5 (P<0.005 by Student's two-tailed t-test) compared with the influenza haemagglutinin (flu HA)-specific control, although not at day 3 (P=0.1). (b) In an equivalent experiment, mAb LT-6E8 did not affect the replication of gp70− MuHV-4 (P=0.2). mAb 58-16D2 (50 μg), which recognizes a different domain of gp70, also significantly reduced the 5 day post-infection titres of wild-type (wt; P<0.00002) but not gp70− (P=0.8) MuHV-4. (c) BALB/c mice were infected i.n. with MuHV-4 (3×104 p.f.u.) and at the same time given mAb LT-6E8 i.p. in differing amounts. Infectious virus titres in lungs were determined by plaque assay 5 days later. Each point shows the titre for one mouse; × shows mean values. All LT-6E8 amounts reduced virus titres compared with the no-antibody control (P<0.003); 500 μg was marginally more effective than 56 or 19 μg (P<0.04), but no more effective than 167 μg (P=0.3). (d) BALB/c mice were infected i.n. with MuHV-4 (3×104 p.f.u.) and at the same time given mAb LT-6E8 (500 μg) or mAb 230-4A2 (500 μg) i.p., both together or no antibody. Infectious virus titres in lungs were determined by plaque assay 5 days later. All mAb treatments reduced virus titres significantly compared with the no-antibody control (P<0.02). Both LT-6E8 and 230-4A2 together were more effective than LT-6E8 alone (P<0.03), but not more effective than 230-4A2 alone (P=0.8). (e) C57BL/6 mice were infected i.n. as in (a), and the effect of immune serum (200 μl) was compared with that of the neutralizing gB-specific IgM mAb MG-2C10 (200 μg). Immune serum reduced virus titres significantly (P<0.0001), whereas MG-2C10 had no effect (P=0.7). (f) Mice were infected i.n. as in (a) and at the same time given antibody or not i.p. (results of two separate experiments are shown). Infectious virus in lungs was titrated by plaque assay 5 days later. mAbs 3F7 (IgG2a, anti-gN, non-neutralizing, 40 μg) and SC-9A5 (IgG3, anti-gB, neutralizing, 500 μg) reduced virus titres (P<0.002), whereas mAb BN-3A4 (IgG1, anti-gp150, non-neutralizing, 700 μg) did not (P=0.9).