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. 2009 Nov;90(Pt 11):2592–2603. doi: 10.1099/vir.0.014266-0

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Copyright © 2009, SGM

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — Antibody-mediated protection by non-neutralizing mAbs and by immune serum is IgG Fc receptor-dependent. (a) FcRγ^−/−FcγRII^−/− mice (IgG FcR^−/−) were compared with 129Sv (IgG FcR^+/+) controls for antibody-dependent protection against MuHV-4. Mice were infected i.n. (3×10⁴ p.f.u.) and at the same time given antibody or not i.p. Infectious virus in lungs was titrated 5 days later by plaque assay. Each point shows the titre for one mouse; × shows mean values. mAbs 3F7 (40 μg) and LT-6E8 (500 μg) both reduced virus titres significantly in FcR^+/+ (P<0.02 by Student's two-tailed t-test) but not FcR^−/− (P=0.3) mice. (b) IgG FcR^−/− or C57BL/6 (IgG FcR^+/+) mice were infected i.n. as in (a) and at the same time given i.p. 500 μg LT-6E8 or DW-6F6 (anti-influenza haemagglutinin). Lungs were titrated for infectious virus 5 days later. mAb LT-6E8 reduced titres significantly in IgG FcR^+/+ (P<0.05) but not IgG FcR^−/− (P=0.5) mice compared with the control. (c) IgG FcR^−/− and 129Sv IgG FcR^+/+ mice were compared by lung virus titre 5 days after i.n. MuHV-4 infection and i.p. injection of either nothing (virus only), immune serum (200 μl) or mAb 58-16D2 (50 μg). Immune serum reduced virus titres significantly in IgG FcR^+/+ (P<0.001) but not IgG FcR^−/− (P=0.3) mice. mAb 58-16D2 also reduced virus titres significantly in IgG FcR^+/+ (P<0.02) but not IgG FcR^−/− mice. (d) IgG FcR^−/− and C57BL/6 IgG FcR^+/+ mice were compared by lung virus titre 5 days after i.n. MuHV-4 infection and i.p. injection of either nothing (virus only) or immune serum (200 μl). Immune serum again reduced virus titres significantly in IgG FcR^+/+ (P<0.0002) but not IgG FcR^−/− (P=0.08) mice.