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. 2009 Nov;90(Pt 11):2592–2603. doi: 10.1099/vir.0.014266-0

Fig. 6.

Fig. 6.

Antibody-mediated protection by neutralizing mAbs is also largely IgG Fc receptor-dependent. (a) C57BL/6 (IgG FcR+/+) or FcRγ−/−FcγRII−/− (IgG FcR−/−) mice were infected with MuHV-4 i.n. (3×104 p.f.u.) and given antibody or not i.p. Five days later, infectious virus in lungs was titrated by plaque assay. Each point shows the titre for one mouse; × shows mean values. IgG FcR+/+ mice showed a significant reduction in virus replication by mAbs SC-9A5 (500 μg) and 3F7 (40 μg) (P<0.001 by Student's two-tailed t-test), but not by mAb T7F5 (200 μg) (P=0.2). None of the mAbs reduced virus replication significantly in IgG FcR−/− mice. (b) A repeat experiment again showed a significant reduction in IgG FcR+/+ lung titres by mAbs 3F7 (40 μg) and SC-9A5 (500 μg) (P<0.0003). This time the reduction in IgG FcR−/− lung titres by SC-9A5, whilst lower than that of IgG FcR+/+ (3.7-fold rather than 17-fold), was also significant (P<0.0003), as was that by 3F7 (P<0.02). (c) IgG FcR+/+ and IgG FcR−/− mice were further compared for virus titre reductions by the neutralizing gH–gL-specific mAbs T2C12 (500 μg) and 230-5B2 (200 μg), using the same infection protocol as in (a). Both mAbs reduced lung virus titres significantly in IgG FcR+/+ (P<0.005) but not IgG FcR−/− (P>0.13) mice.