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. 2009 Nov;90(Pt 11):2622–2633. doi: 10.1099/vir.0.014175-0

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Copyright © 2009, SGM

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Serological response to AAV-2 in Irish blood donors. (a) AAV-2-specific total IgG in human donor plasma (n=45). Specific IgG was determined by indirect AAV-2 antigen ELISA, normalized by comparison with standard positive and negative samples. The negative cut-off (dashed line) was defined at 2 sd above the concentration of a known seronegative standard. (b–e) AAV-2-specific IgG subclasses present in IgG-positive samples (n=41). IgG1(b), IgG2 (c), IgG3 (d) and IgG4 (e) determined by AAV-2-specific direct antigen ELISA for triplicate samples and quantified by comparison with known reference sera.