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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Haemophilia. 2010 May;16(102):44–55. doi: 10.1111/j.1365-2516.2008.01905.x

Figure 3. T-cell epitope mapping for haemophilic subject IV-3.

Figure 3

Total CD4+ cells (A) and CD4+ cells depleted of CD4+CD25+ cells (B) were stimulated with pooled peptides spanning the FVIII C2 domain sequence. Fifteen days later, the cells were incubated with PE-labeled DR1104 tetramers loaded with FVIII pooled peptides and antibodies as described in Methods. Decoding of DR1104-restricted responses to peptide pool 2 using tetramers loaded with individual peptides comprising the pool was carried out 22 days after stimulation of CD4+CD25+-depleted CD4+ cells with peptide pool 2 (C).