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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Haemophilia. 2010 May;16(102):44–55. doi: 10.1111/j.1365-2516.2008.01905.x

Figure 6. Tetramer staining of T-cell clones isolated from haemophilic subject IV-3.

Figure 6

A. T-cell clones #15, 19, 11, and 12 were stimulated with HLA-mismatched PBMCs and PHA. Thirteen days later, the clones were incubated with PE-labeled DR1104 tetramers loaded with peptide FVIII2202-2221 and APC-labeled anti-human CD4 IgG. B. Two of the clones were incubated with DR1104 tetramers loaded with an irrelevant peptide (FVIII2186-2205) as a negative control.