Fig. 4.

Mutations in the C-terminal region of the B5 ST affected plaque size, EEV titre and sensitivity to HP. (a) Alanine substitutions in the B5 ST. (b) Western blot of the B5 and D8 proteins. Infected cell lysates were prepared, processed and analysed by immunoblotting as in Fig. 3b except that anti-D8 mAb was used. (c) Plaque size analysis as in Fig. 3. (d) EEV production as in Fig. 3e. The IMV NAb used was the IMV-N-serum (diluted 1 : 100) rather than mAb 2D5. (e) Effect of HP on EEV membrane disruption. See Fig. 2(b) for details except that IMV-N serum (1 : 100) was used instead of 2D5.