Table 1.
Hemolytic activity of the most active sponge extracts.
| Aqueous extracts | Organic extracts1 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sponge species | S# | Amount in the assay (μg/mL) | Hemolytic activity | Amount in the assay (μg/mL) | Hemolytic activity | ||||||
| Unheated | Heated | Unheated | Heated | A | B | M | A | B | M | ||
| Agelas clathrodes | 81 | 32 | +++ | ||||||||
| Ircinia felix | 59 | 2.6 | 43 | 88 | +++ | +++ | +++ | ||||
| Lissodendoryx colombiensis | 110 | 25 | +++ | ||||||||
| Pandaros acanthifolium | 76 | 60.5/10.4* | +++ | ||||||||
| Spheciospongia vesparium | 69 | 408/143* | +++ | ||||||||
| Topsentia ophiraphidites | 99 | 2.5/0.22* | 2.5/0* | +++ | +++ | 157 | +++ | ||||
1
organic extracts: A (acetone), B (butanol), M (methanol).
+++, hemolytic activity (t50 between 0 and 5 min). t50 = half-time of hemolysis, e.g. the time in which 50% of erythrocytes are lysed.
*
protein.
S# sample number.