Figure 4.
Effects of magnetic targeting on short-term cell retention and long-term cell engraftment. A, female animals (n=6) were sacrificed 24 hours after cell injection. Donor male cells persistent in the female hearts were detected by quantitative PCR for the SRY gene. B, similar PCR experiment performed 3 weeks after injection. C and D, CDCs were labeled with flash-red-conjugated SPMs and then injected into animals with (D) or without (C) magnetic targeting. At 3 weeks after injection, representative hearts from both groups (n=3) were harvested and imaged for detection of flash-red fluorescence. More fluorescence is evident in the Fe-CDC+Magnet heart. E, fluorescence intensities (photon/s) from a fixed region of interest (ROI) measured with the Xenogen software.