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. Author manuscript; available in PMC: 2010 Jun 14.

Published in final edited form as: Mol Microbiol. 2005 Oct;58(1):102–115. doi: 10.1111/j.1365-2958.2005.04811.x

Fig. 4 — Effect of various spo mutations on σ^G and σ^K activity. Strains were induced to sporulate via resuspension with samples removed and β-galactosidase activity measured at hourly intervals.

A. Activity of σ^G-dependent sspB-lacZ at 37°C. Wild type (*; KP6); spoIIQ (○ KP950); spoIVB (□; KP957); spoIIIAG-H (◇; KP952); spoIIP (▽; KP951).

B–D. Activity of the σ^K-dependent cotD–lacZ fusion at 37°C.

B. Wild type (*; KP946), spoIIIG (△; KP947), spoIIIG bofA (▲; KP948), spoIIQ (○; KP953), spoIIQ bofA (●; KP955).

C. Wild type (*; KP946), spoIIP (▽; KP958), spoIIP bofA (▼; KP959), spoIVB (□; KP954); spoIVB bofA (■; KP956).

D. Wild type (*; KP946), spoIIIAG-H (◇; KP962); spoIIIAG-H bofA (◆; KP963), spoIVB (□; KP954); spoIVB bofA (■; KP956).

E–F. Activity of the σ^K-dependent cotD–lacZ fusion at 30°C.

E. Wild type (*; KP946), spoIIIG (△; KP947), spoIIIG bofA (▲; KP948), spoIVB (□; KP954); spoIVB bofA (■; KP956).

F. spoIIQ (○; KP953), spoIIQ bofA (●; KP955), spoIIP (▽; KP958), spoIIP bofA (▼; KP959), spoIIIAA-H (◇; KP960); spoIIIAA-H bofA (◆; KP961). Identical results were obtained with other engulfment mutants (spoIID or spoIIM).