Fig. 8.

Pyrazole prevents the activation of NF-kB by TNFα. Levels of IkBalpha (a), NF-kB DNA binding (b) and content of SOD2 (manganese superoxide dismutase) after treatment with saline, pyrazole alone, TNFα alone, or TNFα plus pyrazole or TNFα plus pyrazole plus the antioxidant N-acetylcysteine (NAC). Results show the activation of NF-kB by TNFα alone (decrease in IkBalpha, increased DNA binding, increased content of the antioxidant enzyme MnSOD) is prevented when TNFα plus pyrazole are administered. The TNFα activation of NF-kB is restored when NAC is administered to TNFα plus pyrazole treated mice