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Binding of elf-125I to EFR and chimeric receptors. A, Western blot with extracts from leaves expressing EFR or the different chimeric receptors stained with anti-GFP antibodies. B, binding of elf-125I in the absence (total binding, filled bars) or presence of 1 μm elf18 (nonspecific binding, open bars). Data shown represent two replicates with bars indicating the mean. C, competition of specific binding with different concentrations of unlabeled elf18.
