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. 2010 Apr 26;285(25):19051–19059. doi: 10.1074/jbc.M110.123620

FIGURE 3.

FIGURE 3.

A and B, activation of AMPK signaling by AICAR suppresses stearate (C18:0) (A)- or LPS (B)- induced TNF-α mRNA expression in macrophages. RAW264.7 macrophages were pre-treated with various concentrations of AICAR as indicated, and then stimulated with LPS (100 ng/ml) or stearate (C18:0) (500 μm) for 4 h. TNF-α mRNA was measured by real time RT-PCR. C and D, activation of AMPK by CA-α1AMPK or AICAR inhibits stearate (C)- or LPS (D)-induced TNF-α expression. RAW264.7 macrophages were transfected with pcDNA3.1, DN-α1AMPK, or CA-α1AMPK, and then treated with vehicle (BSA for stearate and H2O for LPS), LPS (100 ng/ml), stearate (500 μm), with or without AICAR (2 mm) as indicated for 4 h. E, activation of AMPK by AICAR or CA-α1AMPK attenuates TNF-α secretion by macrophages. RAW264.7 macrophages transfected with pcDNA3.1, DN-α1AMPK, or CA-α1AMPK or treated with AICAR (2 mm) were stimulated with stearate (250 μm) for 24 h. All data are expressed as mean ± S.E., n = 6–8, *, p < 0.05. A.U., arbitrary units.