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. 2010 Apr 23;285(25):19068–19075. doi: 10.1074/jbc.M109.040477

FIGURE 2.

FIGURE 2.

The effects of MMC on the binding and degradation of 18S rRNA in vivo. Total RNA was prepared as noted and electrophoresed on a denaturing 1.5% agarose gel. The effects on 18S and 28S rRNA were observed and noted. a, total RNA isolated from MCF-7 cells treated with MMC (0–1 μm) under normoxic conditions for 1.5 h in vivo. b, total RNA isolated from MCF-7 cells treated with MMC (0–1 μm) under hypoxic conditions for 1.5 h in vivo. The 18S and 28S rRNA bands appear to “shift” and correspond to a calculated increase in mass of ∼150 bases.