FIGURE 4.

MMC decreases protein translation in a cell-free system. Rabbit reticulocyte lysates were incubated with 0–10 μm MMC along with mRNA encoding firefly luciferase and protein translation allowed to proceed for 90 min. Additionally, positive control reactions were run using 0.1–1 μg/μl cycloheximide (CX). Reactions were carried out under normoxic (a) or hypoxic (b) conditions. Luciferase activity was measured, with the luminescence intensity correlating with amount of luciferase protein present. Values are expressed as percent luminescence compared with an untreated vehicle control. Bars are means ± S.E. ***, p < 0.01 versus untreated control.