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. 2010 Apr 12;285(25):19085–19091. doi: 10.1074/jbc.M109.100032

FIGURE 2.

FIGURE 2.

SNM1A physically interacts with Ub and PCNA through its UBZ domain and PIP box. A, GST and GST-Ub were used to pull down human wild-type and mutant GFP-SNM1A overexpressed in HEK 293T cells. PIP(YFAA) is the PIP Box mutant with mutation of YF (562/563) to AA. UBZ(CCAA) is the UBZ mutant with the mutation CC (122/125) to AA. UBZ(C125F) is another UBZ mutant with the mutation C125F. B, GST, GST-PCNA, and GST-PCNA-Ub agarose were used to pull down human wild-type and mutant GFP-SNM1A overexpressed in HEK 293T cells. EV is the empty vector.