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Expression of WT-REF1 and DN-REF1 and efficacy of BER enzyme knockdowns. MCF-7 cells were transfected with WT-REF1 (A) or DN-REF (B) or treated with REF1-siRNA (C), OGG1-siRNA (D), or NTH1-siRNA (E), as described above. The cells were then harvested for Western blotting to detect the indicated BER enzyme or actin.
