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. 2010 Apr 27;285(25):19106–19115. doi: 10.1074/jbc.M109.099770

FIGURE 1.

FIGURE 1.

HRG-β1 regulates the transcription of FABP5 in MCF-7 cells. a, MCF-7 cells were serum-starved for 24 h before treatment with HRG-β1 (30 ng/ml) for the indicated times. FABP5 mRNA levels were assessed by Q-PCR. Data are the means ± S.D. (n = 3). b, serum-starved MCF-7 cells were treated with HRG-β1 (30 ng/ml) for the denoted times before lysis. Lysates were analyzed by immunoblots. c, serum-starved MCF-7 cells were treated with actinomycin D (actD, 5 μg/ml) before the addition of HRG-β1 (30 ng/ml) for the denoted times. FABP5 mRNA levels were measured by Q-PCR. Data are the mean ± S.D. (n = 3). d, serum-starved MCF-7 cells were treated with cycloheximide (CHX, 20 μg/ml) for 10 min before the addition of HRG-β1 (30 ng/ml, 4 h). Levels of FABP5 mRNA were measured by Q-PCR. Data are the mean ± S.D. (n = 3).