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. 2010 Apr 27;285(25):19106–19115. doi: 10.1074/jbc.M109.099770

FIGURE 6.

FIGURE 6.

Induction of MCF-7 cell proliferation by HRG-β1 is mediated by FABP5 and PPARβ/δ. a and d, MCF-7 cells were infected with lentiviruses harboring shRNA for FABP5 (a) or PPARβ/δ (d). Insets, immunoblots demonstrate down-regulation of denoted proteins 3 days after infection. Three days after infection, cells were serum-starved before the addition of HRG-β1 for 4 h. Levels of PDK1 mRNA were measured by Q-PCR. Data are the mean ± S.D. (n = 3). *, p < 0.05 versus HRG-β1 control. ctr, control. b, c, and e, MCF-7 cells were infected with an empty lentivirus or lentivirus harboring shRNA for FABP5 (b and c) or PPARβ/δ (e). 3 days after infection, cells were treated with HRG-β1 for 24 h. Cell proliferation was assessed by bromodeoxyuridine (BRDU) incorporation (b and e) and by cell counting (c). Data are the mean ± S.D. p = 0.01 (*), p = 0.05 (**), and p = 0.02 (***) versus HRG-β1-treated controls.