TABLE 1.

Characteristics of PC6-3 cell lines with altered Tau expression

Cell line	Parental line	Stably expressed plasmid	Tau expression levels
PC6-3		None	Control
D5	PC6-3	pRc/CMVn123c	0N3R human Tau was expressed 4-fold higher than endogenous Tau in undifferentiated cells and 2-fold higher after differentiation
rTau4	PC6-3	pNTO-Rtau	No Tau was detected in undifferentiated or differentiated cells
rTau7	PC6-3	pNTO-Rtau	No Tau was detected in undifferentiated cells and Tau expression was 15% of control cell level after differentiation
EV	PC6-3	pNTO Vector	Tau levels were equivalent to parental PC6-3 cells, with or without NGF

Tau expression levels were determined by quantitative Western blotting, with or without NGF (3 days at 100 ng/ml).